CRL-1682 AsPC-1 人轉(zhuǎn)移胰腺腺癌細(xì)胞
ATCC® Number: CRL-1682?
Designations: AsPC-1
Depositors: MH Tan
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology:
CRL-1682 AsPC-1 人轉(zhuǎn)移胰腺腺癌細(xì)胞
Source: Organ: pancreas
Disease: adenocarcinoma
Derived from metastatic site: ascites
Cellular Products: carcinoembryonic antigen (CEA); human pancreas associated antigen; human pancreas specific antigen; mucin
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Applications: transfection host (Roche FuGENE® Transfection Reagents)
DNA Profile (STR): Amelogenin: X
CSF1PO: 10,13
D13S317: 9,12
D16S539: 11
D5S818: 12
D7S820: 12,13
THO1: 7,9.3
TPOX: 8,10
vWA: 17
Age: 62 years
Gender: female
Ethnicity: Caucasian
Comments: The line was derived from nude mouse xenografts initiated with cells from the ascites of a patient with cancer of the pancreas.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol:
CRL-1682 AsPC-1 人轉(zhuǎn)移胰腺腺癌細(xì)胞Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 2152: Chen WH, et al. Human pancreatic adenocarcinoma: in vitro and in vivo morphology of a new tumor line established from ascites. In Vitro 18: 24-34, 1982. PubMed: 7182348
22552: Tan MH, et al. Differential localization of human pancreas cancer-associated antigen and carcinoembryonic antigen in homologous pancreatic tumoral xenograft. J. Natl. Cancer Inst. 67: 563-569, 1981. PubMed: 6944528
22737: Loor R, et al. Use of pancreas-specific antigen in immunodiagnosis of pancreatic cancer. Clin. Lab. Med. 2: 567-578, 1982. PubMed: 6754226
23079: Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903
26140: Tan MH, Chu TM. Characterization of the tumorigenic and metastatic properties of a human pancreatic tumor cell line (AsPC-1) implanted orthotopically into nude mice. Tumor Biol. 6: 89-98, 1985. PubMed: 4023565
CRL-1682 AsPC-1 人轉(zhuǎn)移胰腺腺癌細(xì)胞
