CRL-1646 FO 小鼠骨髓瘤細胞
ATCC® Number: CRL-1646? Price: $355.00
Designations: FO
Depositors: SF de St. Groth
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (mouse)
Morphology: lymphoblast
Source: Disease: plasmacytoma; myeloma
Strain: BALB/c
Cell Type: B lymphoblast;
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Antigen Expression: H-2d
Comments: The line was line created by self fusing Sp2/0 myeloma cells using Sendai virus.
The cells are resistant to 8-azaguanine and do not produce immunoglobulin.
The line can be used a fusion partner for making B cell hybridomas.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 0.5 to 1 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Doubling Time: 8 to 10 hrs
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 1092: de St.Groth SF, Scheidegger D. Production of monoclonal antibodies: strategy and tactics. J. Immunol. Methods 35: 1-21, 1980. PubMed: 7009747
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