CCL-127 IMR-32 人神經母細胞瘤細胞
ATCC® Number: CCL-127?
Designations: IMR-32
Depositors: WW Nichols
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: fibroblast; neuroblast
Source: Organ: brain
Disease: neuroblastoma
Cell Type: neuroblast;
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Isolation: Isolation date: April, 1967
Applications: transfection host (technology from amaxa)
Virus Susceptibility: vesicular stomatitis (Indiana); herpes simplex; vaccinia; coxsackievirus B3; poliovirus 3 (poorly)
Virus Resistance: echovirus 11
Reverse Transcript: negative
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 9
D16S539: 8
D5S818: 11,12
D7S820: 9,10
THO1: 7,9.3
TPOX: 11
vWA: 15
Cytogenetic Analysis: Stable male karyotype with stemline number of 49. Two large marker chromosomes with submedian centromeres. A deletion in one number 1 chromosome: One number 16 chromosome missing; two extra chromosomes in C group. Sublines with 50 and 48 chromosomes differ from those with 49 chromosomes by having an extra or missing C group chromosome respectively.
Isoenzymes: G6PD, B
Age: 13 months
Gender: male
Ethnicity: Caucasian
Comments: The IMR-32 cell line was established by W.W. Nichols, J. Lee and S. Dwight in April, 1967 from an abdominal mass occurring in a 13-month-old Caucasian male. [22190]
The tumor was diagnosed as a neuroblastoma with rare areas of organoid differentiation.
Two cell types are present.
Predominant is a small neuroblast-like cell.
The other is a large hyaline fibroblast.
The cell line was submitted to the American Type Culture Collection in the 36th passage. It has been demonstrated that the cells can be propagated successfully beyond the 80th serial subculture.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.Maintain cultures at a cell concentration between 4 X 10 exp4 and 4 X 10 exp5 cells/cm2.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Doubling Time: approximately 20 hrs.
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22190: Tumilowicz JJ, et al. Definition of a continuous human cell line derived from neuroblastoma. Cancer Res. 30: 2110-2118, 1970. PubMed: 5459762
32287: Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024
32459: Maestrini E, et al. A family of transmembrane proteins with homology to the MET-hepatocyte growth factor receptor. Proc. Natl. Acad. Sci. USA 93: 674-678, 1996. PubMed: 8570614
