HTB-33 ME-180 人**頸表皮癌細胞
ATCC® Number: HTB-33?
Designations: ME-180
Depositors: JA Sykes
Biosafety Level: 2 [CELLS CONTAIN PAPOVAVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial
Source: Organ: cervix
Disease: epidermoid carcinoma
Derived from metastatic site: omentum
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Virus Susceptibility: susceptibility of ME-180 to a wide range of viruses as compared with HeLa (ATCC CCL-2), was reported in J. Natl. Cancer Inst. 45:107-122, 1970
Tumorigenic: Yes
Oncogene: p53 +; pRB +
Antigen Expression: Blood Type A; Rh+; HLA A1, A11, B5(+/-), B40
DNA Profile (STR): Amelogenin: X
CSF1PO: 11
D13S317: 11,13
D16S539: 12,13
D5S818: 12
D7S820: 9,10
THO1: 8,9.3
TPOX: 8,10
vWA: 15,17
Cytogenetic Analysis: hyperdiploid to hypohexaploid with abnormalities including dicentrics, fragmentation and markers; unstable; (P63) hypotriploid with abnormalities including dicentrics and acrocentric fragments, large submetacentric and large subtelocentric markers
Isoenzymes: AK-1, 1
ES-D, 1-2
G6PD, B
GLO-I, 2
PGM1, 1-2
PGM3, 1
Age: 66 years
Gender: female
Ethnicity: Caucasian
Comments: This line was derived from a highly invasive squamous cell carcinoma with irregular cell clusters and no significant keratinization.
Desmosomal attachments were observed between cells in monolayer culture and cytoplasmic tonofilaments were noted.
Contamination with Mycoplasma arginini was detected and eliminated in 1970.
Growth of ME-180 cells is inhibited by tumor necrosis factor alpha (TNF alpha).
The cells contain human papillomavirus (HPV) DNA with greater homology to HPV-39 than HPV-18.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2007
recommended serum:ATCC 30-2020
References: 21405: Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111
22520: Sykes JA, et al. Some properties of a new epithelial cell line of human origin. J. Natl. Cancer Inst. 45: 107-122, 1970. PubMed: 4317734
22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
22995: Pater MM, Pater A. Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of the cervix. Virology 145: 313-318, 1985. PubMed: 2992153
23180: Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217
23226: Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212
23238: Reuter S, et al. Characterization of a novel human papillomavirus DNA in the cervical carcinoma cell line ME180. J. Virol. 65: 5564-5568, 1991. PubMed: 1716694
23324: Scheffner M, et al. The state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines. Proc. Natl. Acad. Sci. USA 88: 5523-5527, 1991. PubMed: 1648218
29988: Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105
32285: van Dijk MA, et al. A functional assay in yeas for the human estrogen receptor displays wild-type and variant estrogen receptor messenger RNAs present in breast carcinoma. Cancer Res. 57: 3478-3485, 1997. PubMed: 9270016
32299: St. Geme JW, et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol. 178: 6281-6287, 1996. PubMed:
