CRL-2021 MEG-01 人成巨核細(xì)胞白血病細(xì)胞
ATCC® Number: CRL-2021?
Designations: MEG-01
Depositors: M Tekeuchi, MJ Fitzgerald
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: mixed, adherent and suspension
Organism: Homo sapiens (human)
Morphology: lymphoblast
Source: Disease: chronic myelogenous leukemia (CML)
Cell Type: megakaryoblast;
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Applications: transfection host (Nucleofection technology from Lonza)
Antigen Expression: CD41 +; CD61 +; CDw14 +
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10
D13S317: 8
D16S539: 9
D5S818: 13
D7S820: 11
THO1: 7
TPOX: 8,11
vWA: 16
Cytogenetic Analysis: hyperdiploid; modal number = 56 to 58; the Philadelphia chromosome (Ph1) is present
Age: 55 years
Gender: male
Comments: The MEG-01 cell line was derived in 1983 at the Nagoya University School of Medicine, Nagoya, Japan from bone marrow cells taken from a patient in megakaryoblastic crisis of CML.
The cells are positive for cytoplasmic Factor VIII and surface GPIIb/IIIa, periodic acid - Schiff (PAS) reaction, alpha naphthyl acetate esterase and acid phosphatase.
They are negative for myeloperoxidase, alpha naphthyl butyrate esterase, naphthol AS-D chloroacetate esterase and alkaline phosphatase.
The stain positively with monoclonal antibodies BA-1 (anti B cell, granulocyte), HPL-3 (anti gpIIb/IIIa) and 20.3 (anti monocyte, platelet).
They are negative for other lymphoid and myeloid seris antigens.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Subcultures are prepared by scraping the adherent cells into the medium. From the resulting suspension dilute cells to a concentration 1-2 X 10exp5 cells/ml into fresh medium in a new flasks. Keep culture below approximate density of 10exp6 cells/ml.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Subcultures are prepared by scraping the adherent cells into the medium, and diluting the resulting suspension into fresh medium in new flasks.
Doubling Time: 36 to 48 hrs
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 23348: Ogura M, et al. Establishment of a novel human megakaryoblastic leukemia cell line, MEG- 01, with positive Philadelphia chromosome. Blood 66: 1384-1392, 1985. PubMed: 2998511
