NCI-H1688 人小細胞肺癌細胞
ATCC® Number: CCL-257? Price: $417.00
Designations: NCI-H1688 [H1688]
Depositors: AF Gazdar, JD Minna
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial
Source: Organ: lung
Disease: carcinoma; classic small cell lung cancer
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DNA Profile (STR): Amelogenin: X
CSF1PO: 12
D13S317: 8
D16S539: 13
D5S818: 10
D7S820: 12
THO1: 6,8
TPOX: 11
vWA: 17
Age: 50 years
Gender: male
Ethnicity: Caucasian
Comments: This line was derived from a liver metastasis taken from a patient prior to therapy.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove spent medium, and add fresh 0.25% trypsin, 0.03% EDTA solution for 2 to 3 minutes at room temperature.
Remove the trypsin and incubate the flask at 37C for 5 to 10 minutes or until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
