CCL-46 P388D1 小鼠**樣瘤細胞
ATCC® Number: CCL-46?
Designations: P388D1
Depositors: EM Jensen
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (mouse)
Morphology: lymphoblast
Source: Disease: lymphoma
Strain: DBA/2
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Applications: Biological response [92560]
transfection host (Roche FuGENE® Transfection Reagents)
Tumorigenic: Yes
Reverse Transcript: positive
Antigen Expression: H-2, d haplotype; Mus musculus, expressed
Comments: A subclone of this line [see ATCC TIB-63, P388D1(IL-1)] produces high levels of interleukin-1 (IL-1, interleukin 1).Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 2 - 5 X 10 (5) viable cells/ml.
A maximum of 6 X 10 (6) viable cells/ml is obtainable.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 10 to 12 hrs
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2040
Cell culture tested DMSO:ATCC 4-X
References: 22208: . . Am. J. Pathol. 33: 603, 1957.
92560: Standard Practice for Testing for Biological Responses to Particles in Vitro. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 1903-98R03.
