CRL-1580 P3X63Ag8.653 小鼠骨髓瘤細(xì)胞
ATCC® Number: CRL-1580?
Designations: P3X63Ag8.653
Depositors: JF Kearney
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (mouse)
Morphology: lymphoblast
Source: Disease: plasmacytoma; myeloma
Strain: BALB/c
Cell Type: B lymphoblast;
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Applications: transfection host (Roche FuGENE® Transfection Reagents)
Antigen Expression: H-2d
Comments: The cells are resistant to 8-azaguanine and are HAT sensitive.
They can be used as fusion partners for producing hybridomas.
The cells have been reported to be cholesterol auxotrophs due to a deficiency in 3-ketosteroid reductase activity.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start new cultures at 4 X 10(5) viable cells/ml and subculture before the cell density reaches 2 X 10(6) cells/ml.
Medium Renewal: Every 3 to 4 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 1075: Kearney JF, et al. A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. J. Immunol. 123: 1548-1550, 1979. PubMed: 113458
23366: Sato JD, et al. Effects of proximate cholesterol precursors and steroid hormones on mouse myeloma growth in serum-free medium. In Vitro Cell. Dev. Biol. 24: 1223-1228, 1988. PubMed: 3209588
32914: Oppermann M, et al. Monoclonal antibodies reveal receptor specificity among G-protein-coupled receptor kinases. Proc. Natl. Acad. Sci. USA 93: 7649-7654, 1996. PubMed: 8755530
