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T-47D
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I Keydar
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Biosafety Level:
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1
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frozen
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See Propagation
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adherent
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Homo sapiens (human)
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epithelial
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Organ: mammary gland; breast
Tissue: duct
Disease: ductal carcinoma
Derived from metastatic site: pleural effusion
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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transfection host (Nucleofection technology from Lonza
Roche FuGENE® Transfection Reagents)
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calcitonin, expressed
androgen receptor, expressed
estrogen receptor, expressed
progesterone receptor, expressed
glucocorticoid receptor, positive, expressed
prolactin, expressed
calcitonin; androgen receptor, positive; progesterone receptor, positive; glucocorticoid; prolactin; estrogen receptor, positive
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Amelogenin: X
CSF1PO: 11,13
D13S317: 12
D16S539: 10
D5S818: 12
D7S820: 11
THO1: 6
TPOX: 11
vWA: 14
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This is a hypotriploid human cell line. The modal chromosome number is 65 occurring at 50% and polyploidy at 0.8%. 18 marker chromosomes are common to most cells, of which 7 are paired and 11 are single-copied. The t(8q14q), t(9q17q), t(10q17p) are among 7 paired markers common to most cells. N7, N9, and N10 are absent and N11 is generally present in 4 copies. DM's occurred, but infrequently. Q-band examination did not show the presence of a Y chromosome.
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AK-1, 1
ES-D, 2
G6PD, B
GLO-I, 1-2
PGM1, 1
PGM3, 1
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54 years
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female
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 0.2 Units/ml bovine insulin; fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
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Protocol:
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Remove and discard culture medium.
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Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
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Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
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Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
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Add appropriate aliquots of the cell suspension to new culture vessels.
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Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week
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Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
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32 hrs
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Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
purified DNA:ATCC 45528
purified DNA:ATCC 45529
purified DNA:ATCC HTB-133D
purified RNA:ATCC HTB-133R
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