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      • 產品名稱：AE-2  小鼠雜交瘤細胞抗AChE
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      • 產品型號：HB-73
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      • 產品廠商：美國標準生物品收藏中心（ATCC）
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      • 產品價格：0
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      • 折扣價格：0
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      • 產品文檔：
                          
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            AE-2  小鼠雜交瘤細胞抗AChE,原代細胞|細胞系|細胞株|菌種;細胞庫管理規范，提供的細胞株背景清楚，提供參考文獻和培養條件!
        
      
    
      

    
      
        
      
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            AE-2  小鼠雜交瘤細胞抗AChE
      

      
	
		
      
			
				ATCC® Number:  
			
			
				HB-73?     
			
			
				Price:  
			
			
				$399.00 
			
		
      
		
      
			
				
      
					
						
      
							
								Designations:  
							
							
								AE-2 
							
						
      
						
      
							
								Depositors:  
							
							
								 DM Fambrough  
							
						
      
						
      
							
								Isotype:  
							
							
								IgG1  
							
						
      
						
      
							
								Biosafety Level: 
							
							
								1 
							
						
      
						
      
							
								Shipped:  
							
							
								frozen  
							
						
      
						
      
							
								Medium & Serum:  
							
							
								See Propagation 
							
						
      
						
      
							
								Growth Properties: 
							
							
								suspension 
							
						
      
						
      
							
								Organism: 
							
							
								Mus musculus (B cell); Mus musculus (myeloma) (mouse (B cell); mouse (myeloma)) 
							
						
      
						
      
							
								Morphology: 
							
							
								lymphoblast
      

      
       
							      		
						
      
						
      
							
								Source: 
							
							
								Cell Type: hybridoma: B lymphocyte; 
							
						
      
						
      
							
								Cellular Products: 
							
							
								immunoglobulin; monoclonal antibody; against human acetylcholinesterase 
							
						
      
						
      
							
								Permits/Forms: 
							
							
								In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. 
							
						
      
						
      
							
								 
							
						
      
						
      
							
								Comments: 
							
							
								Animals were immunized with purified human erythrocyte acetylcholinesterase. 
      
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. 
      
The antibody cross-reacts with human and monkey neuromuscular junctions. 
      
Tested and found negative for ectromelia virus (mousepox).       
							      		
						
      
						
      
							
								Propagation:  
							
							
								ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
      
       
							      		
						
      
						
      
							
								Subculturing:  
							
							
								Medium Renewal: Every 2 to 3 days 
      
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.       
							      		
						
      
						
      
							
								References: 
							
							
								939: Fambrough DM, et al. Acetylcholinesterase of human erythrocytes and neuromuscular junctions: homologies  
							
						
      
					      
				
      
			      		
		
      
	      

      
        
      
    
      

      
 
    
      

      

                            
      
    
      
    
      
        
      

  
      


          
      
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