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      • 產品名稱：B16F10 小鼠黑色素瘤高轉移細胞
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      • 產品型號：CRL-6475
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      • 產品廠商：美國標準生物品收藏中心（ATCC）
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      • 產品價格：0
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      • 折扣價格：0
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      • 產品文檔：
                          
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            CRL-6475 B16F10 小鼠黑色素瘤高轉移細胞,原代細胞|細胞系|細胞株|菌種;細胞庫管理規范，提供的細胞株背景清楚，提供參考文獻和培養條件!
        
      
    
      

    
      
        
      
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            CRL-6475 B16F10 小鼠黑色素瘤高轉移細胞
      

      
	
		
      
			
				ATCC® Number:  
			
			
				CRL-6475?     
			
			
				Price:  
			
			
				$269.00 
			
		
      
		
      
			
				
      
					
						
      
							
								Designations:  
							
							
								B16-F10 
							
						
      
						
      
							
								Biosafety Level:
							
							
								1 
							
						
      
						
      
							
								Shipped:  
							
							
								frozen  
							
						
      
						
      
							
								Medium & Serum:  
							
							
								See Propagation
							
						
      
						
      
							
								Growth Properties: 
							
							
								adherent 
							
						
      
						
      
							
								Organism: 
							
							
								Mus musculus (mouse) 
							
						
      
						
      
							
								Morphology: 
							
							
								melanocyte
      
CRL-6475 B16F10 小鼠黑色素瘤高轉移細胞
      
       
							      		
						
      
						
      
							
								Source: 
							
							
								Organ: skin 
      
Strain: C57BL/6J 
      
Disease: melanoma       
							      		
						
      
						
      
							
								Permits/Forms: 
							
							
								In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. 
							
						
      
						
      
							
								 
							
						
      
						
      
							
								Applications: 
							
							
								transfection host (technology from amaxa) 
							
						
      
						
      
							
								Propagation:  
							
							
								ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
      
Temperature: 37.0°C 
      
Atmosphere: air, 95%; carbon dioxide (CO2), 5%       
							      		
						
      
						
      
							
								Subculturing:  
							
							
								Protocol:  
								
        
									
      1. 
										Remove and discard culture medium.
									
        2. 
									
      2. 
										Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
									
        3. 
									
      3. 
										Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
        
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
									
        4. 
									
      4. 
										Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
									
        5. 
									
      5. 
										Add appropriate aliquots of the cell suspension to new culture vessels.
									
        6. 
									
      6. 
										Incubate cultures at 37°C.
										
          
											
          
Subcultivation Ratio: A subcultivation ratio of 1:10 is recommended 
          
Medium Renewal: Every 2 to 3 days
										
        
									
        7. 
								
      
       
							      		
						
      
						
      
							
								Preservation:  
							
							
								Freeze medium: culture medium, 95%; DMSO, 5% 
      
Storage temperature: liquid nitrogen vapor phase       
							      		
						
      
					      
				
      
			      		
		
      
	      

      
        
      
    
      

      
 
    
      

      

                            
      
    
      
    
      
        
      

  
      


          
      
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