<cite id="pwzsq"><rp id="pwzsq"></rp></cite>


      
欧美视频精品免费覌看,7777久久亚洲中文字幕蜜桃,免费观看的av在线播放,国产一区二区三区免费主播,久久精品aⅴ无码中文字幕,中文字幕日韩有码国产,啊灬啊灬啊灬快灬深学长慢视频,国产片av片永久免费观看 

    
      
        
      
            
      
                
      
                    
                
      
                
                
      
                    
      
                        
      
                            
      
                                
      
                                    
                                
      
                                
      
                                    
                                    
      
                                        
      
                                            
      
                                                
      
        
      
        
      
            
      
                
      
                    
                    
                    
                    
                    
                
      
                
      
                
      
                    
      
                        
      
                            產品詳情
                        
      
                        
      
                            
      
                                
                                
                                
                                
                            
      
                            
      
    
      
    
      
        
      
    
      
            
      

                
      
                    
                    
        
                          
      • 產品名稱：C2C12 小鼠成肌細胞
        • 
                          
      • 產品型號：CRL-1772
        • 
                          
      • 產品廠商：美國標準生物品收藏中心（ATCC）
        • 
                          
      • 產品價格：0
        • 
                          
      • 折扣價格：0
        • 
                          
      • 產品文檔：
                          
        • 




                    
      
                    
        
                           
      • 
                             
        
                               
                             
        
                         
        • 
                    
      
                     
      
                    你添加了1件商品 查看購物車
                    
      

                
      
            
      
    
      

    
      
        
      
            簡單介紹：
        
      
        
      
            CRL-1772 C2C12 小鼠成肌細胞，原代細胞|細胞系|細胞株|菌種；細胞庫管理規范，提供的細胞株背景清楚，提供參考文獻和培養條件！
        
      
    
      

    
      
        
      
            詳情介紹：
        
      
        
      
            CRL-1772 C2C12 小鼠成肌細胞
      

      
	
		
      
			
				ATCC® Number: 
			
			
				CRL-1772?    
			
			
				Price: 
			
			
				$256.00
			
		
      
		
      
			
				
      
					
						
      
							
								Designations: 
							
							
								C2C12
							
						
      
						
      
							
								Biosafety Level:
							
							
								1
							
						
      
						
      
							
								Shipped: 
							
							
								frozen 
							
						
      
						
      
							
								Medium & Serum: 
							
							
								See Propagation
							
						
      
						
      
							
								Growth Properties:
							
							
								adherent
							
						
      
						
      
							
								Organism:
							
							
								Mus musculus (mouse)
							
						
      
						
      
							
								Morphology:
							
							
								myoblast
      
CRL-1772 C2C12 小鼠成肌細胞
      
      
							      		
						
      
						
      
							
								Source:
							
							
								Strain: C3H 
      
Tissue: muscle 
      
Cell Type: myoblast;      
							      		
						
      
						
      
							
								Permits/Forms:
							
							
								In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
							
						
      
						
      
							
								 
							
						
      
						
      
							
								Applications:
							
							
								transfection host (Nucleofection technology from Lonza
      
Roche FuGENE® Transfection Reagents)      
							      		
						
      
						
      
							
								Comments:
							
							
								This is a subclone (produced by H. Blau, et al) of the mouse myoblast cell line established by D. Yaffe and O. Saxel. [22903] 
      
The C2C12 cell line differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins. [22953] 
      
Treatment with bone morphogenic protein 2 (BMP-2) cause a shift in the differentiation pathway from myoblastic to osteoblastic. [23427] 
      
Tested and found negative for ectromelia virus (mousepox).      
							      		
						
      
						
      
							
								Propagation: 
							
							
								ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
      
Temperature: 37.0°C      
							      		
						
      
						
      
							
								Subculturing: 
							
							
								Protocol: IMPORTANT - DO NOT ALLOW CULTURES TO BECOME CONFLUENT. 
      
Cultures must not be allowed to become confluent as this will deplete the myoblastic population in the culture.
      
Myotube formation is enhanced when the medium is supplemented with 10% horse serum instead of fetal bovine serum.
								
        
									
      1. 
										Remove and discard culture medium.
									
        2. 
									
      2. 
										Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
									
        3. 
									
      3. 
										Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
        
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
									
        4. 
									
      4. 
										Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
									
        5. 
									
      5. 
										Add appropriate aliquots of the cell suspension to new culture vessels.
        
Inoculate at a cell concentration between 1.5 X 10 exp5 and 1.0 X 10 exp6 viable cells/75 cm2.
									
        6. 
									
      6. 
										Incubate cultures at 37°C.
									
        7. 
								
      

      
      
							      		
						
      
					      
				
      
			      		
		
      
	      

      
        
      
    
      

      
 
    
      

      

                            
      
    
      
    
      
        
      

  
      


          
      
              姓名：
              
      
                  
              
      
          
      
          
      
              電話：
              
      
                  
              
      
          
      
          
      
                  Email：
                  
      
                      
                  
      
              
      
          
      
              您的需求：
              
      
                  
              
      
          
      
          
      
               
              
      
                  
                  
              
      
          
      

  
      
  

      

    
      

      

                        
      
                    
      
                
      
            
      
        
      
    
      
                                            
      
                                        
      
                                    
      
                                
      
                            
      
                        
      
                    
      
                
      
                
            
      
		 		
      滬公網安備 31010902002429號
      
		 	
      
		 
      
        
      
    
      
 


主站蜘蛛池模板：
激情欧美精品一区二区|
国产免费踩踏调教视频|
尤物AV尤物AV在线观看|
欧洲女人牲交性开放视频|
91视频播放|
色欲天天综合网|
国产精品一区二区三粉嫩|
亚洲激情图片|
日韩巨乳在线观看|
国产在线自在拍91精品黑人|
亚洲精品字幕|
人人肏|
国产在线精品欧美日韩电影|
欧美成人综合|
久久精品国产av一区二区三区
|
黄www|
精品久久久久久亚洲中文字幕
|
无码免费一区二区三区|
亚洲精品9999久久久久无码
|
高清无码人妻|
亚州中文字幕av|
先锋影音成人影音|
新郑市|
无码射肉在线播放视频|
午夜中文在线|
高清无码爆乳潮喷在线观看|
中文字幕一区二区三区日韩精品|
国产精品无码v在线观看|
丁香六月久久婷婷开心|
午夜福利视频|
新闻|
AV福利在线|
【_undefined?-?P站免费版?-?永久免费的福利视频平台】https://17630364268551281430832.nx37lbnqvd.com/column/all/show?t=&tags=%E5%90%8E%E5%85%A5%E9%AA%91%E9%A9%AC&page=2&orderBy=createTime&expanded=1
|
国产普通话对白刺激|
成人A片在线观看|
影音先锋亚洲无一区二区三区|
三区在线视频|
国产精成人品日日拍夜夜|
日韩人妻无码专区一本二|
国产精品香蕉|
亚洲精品久荜中文字幕|