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      • 產(chǎn)品名稱：Calu-6 人肺癌細胞系（未分化癌）
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      • 產(chǎn)品型號：HTB-56
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      • 產(chǎn)品廠商：美國標準生物品收藏中心（ATCC）
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      • 產(chǎn)品價格：0
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      • 折扣價格：0
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            HTB-56 Calu-6 人肺癌細胞系（未分化癌）原代細胞|細胞系|細胞株|菌種；細胞庫管理規(guī)范，提供的細胞株背景清楚，提供參考文獻和培養(yǎng)條件！
        
      
    
      

    
      
        
      
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            HTB-56 Calu-6 人肺癌細胞系（未分化癌）
      

      
	
		
      
			
				ATCC® Number:  
			
			
				HTB-56?     
			
			
				Price:  
			
			
				$329.00 
			
		
      
		
      
			
				
      
					
						
      
							
								Designations:  
							
							
								Calu-6 
							
						
      
						
      
							
								Depositors:  
							
							
								 J Fogh  
							
						
      
						
      
							
								Biosafety Level: 
							
							
								1 
							
						
      
						
      
							
								Shipped:  
							
							
								frozen  
							
						
      
						
      
							
								Medium & Serum:  
							
							
								See Propagation 
							
						
      
						
      
							
								Growth Properties: 
							
							
								adherent 
							
						
      
						
      
							
								Organism: 
							
							
								Homo sapiens (human) 
							
						
      
						
      
							
								Morphology: 
							
							
								epithelial
      

      
       
							      		
						
      
						
      
							
								Source: 
							
							
								Organ: unknown, probably lung 
      
Disease: anaplastic carcinoma       
							      		
						
      
						
      
							
								Permits/Forms: 
							
							
								In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. 
							
						
      
					      
				
      
			      		
		
      
	      

      

      
	
		
      
			
				Propagation:  
			
			
				ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
      
Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
      
Temperature: 37.0°C       
			      		
		
      
		
      
			
				Subculturing:  
			
			
				Protocol:  
				
        
					
      1. 
						Remove and discard culture medium.
					
        2. 
					
      2. 
						Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
					
        3. 
					
      3. 
						Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
        
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
					
        4. 
					
      4. 
						Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
					
        5. 
					
      5. 
						Add appropriate aliquots of the cell suspension to new culture vessels.
					
        6. 
					
      6. 
						Incubate cultures at 37C.
					
        7. 
				
      

      
Interval: every 6 to 8 days 
      
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended 
      
Medium Renewal: 2 to 3 times per week       
			      		
		
      
		
      
			
				Preservation:  
			
			
				Freeze medium: Culture medium, 95%; DMSO, 5% 
      
Storage temperature: liquid nitrogen vapor phase       
			      		
		
      
	      

      

      
        
      
    
      

      
 
    
      

      

                            
      
    
      
    
      
        
      

  
      


          
      
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