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DI TNC1
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CF Deschepper
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Biosafety Level:
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2 [Cells contain polyomavirus DNA sequences ]
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frozen
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Medium & Serum:
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See Propagation
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adherent
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Rattus norvegicus (rat)
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fibroblast
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Organ: brain
Strain: Sprague-Dawley
Tissue: diencephalon
Disease: normal
Cell Type: astrocyte, type 1 phenotype;
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alpha 2 macroglobulin; transferrin
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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These cells are distributed for research purposes only. Dr. Deschepper has requested the following: 1.) No distribution of any of the cells derived from DI TNC1 should be made to third parties. 2.) Any proposed commercial use of these cells should first be negotiated with the Clinical Research Institute of Montreal, 110 Pine Ave West, Montreal, PQ Canada H2W1R7. 3.) In all papers reporting any use of these or derived clones a direct reference will be made to the original publication (Proc. Natl. Acad. Sci. USA 89:6467-6471, 1992).
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No
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neonate
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
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Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove.
Add fresh trypsin solution, (1 to 2 ml) and allow the cells to sit at room temperature (or at 37C) until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
NOTE: The cells will come off in sheets and are difficult to dissociate.
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culture medium 95%; DMSO, 5%
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Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
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23333: Radany EH, et al. Directed establishment of rat brain cell lines with the phenotypic characteristics of type 1 astrocytes. Proc. Natl. Acad. Sci. USA 89: 6467-6471, 1992. PubMed: 1378628
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