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      • 產(chǎn)品名稱：CRL-2285 Super Tube 狗腎細胞
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      • 產(chǎn)品型號：CRL-2285
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      • 產(chǎn)品廠商：美國標準生物品收藏中心（ATCC）
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            CRL-2285 Super Tube 狗腎細胞，原代細胞|細胞系|細胞株|菌種；細胞庫管理規(guī)范，提供的細胞株背景清楚，提供參考文獻和Z優(yōu)培養(yǎng)條件！
        
      
    
      

    
      
        
      
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            CRL-2285 Super Tube 狗腎細胞
      

      
	
		
      
			
				ATCC® Number:  
			
			
				CRL-2285?     
			
			
				  
			
			
				
			
		
      
		
      
			
				
      
					
						
      
							
								Designations:  
							
							
								Super Tube 
							
						
      
						
      
							
								Depositors:  
							
							
								 RJ Klebe  
							
						
      
						
      
							
								Biosafety Level: 
							
							
								1 
							
						
      
						
      
							
								Shipped:  
							
							
								frozen  
							
						
      
						
      
							
								Medium & Serum:  
							
							
								See Propagation 
							
						
      
						
      
							
								Growth Properties: 
							
							
								adherent 
							
						
      
						
      
							
								Organism: 
							
							
								Canis familiaris 
							
						
      
						
      
							
								Morphology: 
							
							
								epithelial
      

      
       
							      		
						
      
						
      
							
								Source: 
							
							
								Organ: kidney 
      
Disease: normal       
							      		
						
      
						
      
							
								Permits/Forms: 
							
							
								In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. 
							
						
      
						
      
							
								 
							
						
      
						
      
							
								Age:  
							
							
								
      
							      		
						
      
						
      
							
								Gender:  
							
							
								female  
							
						
      
						
      
							
								Comments: 
							
							
								Super Tube is a canine epithelial-like cell line established by cloning from the MDCK (ATCC CCL-34) cell line that was derived from a kidney of an apparently normal ***** female cocker spaniel. 
      
Super tube forms large numbers of tubules when maintained at high cell density (cells are reported to form tubules within 3 days when plated at 7 x 10exp5 cells/well of a 24 well plate). 
      
For tubule formation, the cells should be fed twice a day by a complete medium change. 
      
Super Tube has a much lower resting level of cyclic-AMP and responds to forskolin stimulation of adenylate cyclase less readily than the original cell line; it also has a lower transepithelal resistance than both Super Dome (ATCC CRL- 2286) and MDCK.       
							      		
						
      
						
      
							
								Propagation:  
							
							
								ATCC complete growth medium: These cells are grown in a medium containing a 1:1 mixture of Dulbeccos Modified Eagles Medium and Hams F12 medium with 2.5 mM L-glutamine adjusted to contain 15 mM HEPES, 0.5 mM sodium pyruvate, and 1.2 g/L sodium bicarbonate supplemented with 0.05 mM non-essential amino acids and 10 fetal bovine serum.
      
Temperature: 37.0°C       
							      		
						
      
						
      
							
								Subculturing:  
							
							
								Protocol: Remove medium, and rinse with 0.25 trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. 
      
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended 
      
Medium Renewal: Every 2 to 3 days       
							      		
						
      
						
      
							
								Preservation:  
							
							
								Freeze medium: Complete growth medium 95; DMSO, 5 
      
Storage temperature: liquid nitrogen vapor temperature       
							      		
						
      
						
      
							
								Related Products: 
							
							
								Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006
      
recommended serum:ATCC 30-2020
      
parental cell line:ATCC CCL-34
      
derived from same cell line:ATCC CRL-2286       
							      		
						
      
						
      
							
								References: 
							
							
								38915: Klebe RJ, et al. Cyclic-AMP deficient MDCK cells form tubules. J. Cell. Biochem. 59: 453-462, 1995. PubMed: 8749715 
							
						
      
					      
				
      
			      		
		
      
	      

      
        
      
    
      

      
 
    
      

      

                            
      
    
      
    
      
        
      

  
      


          
      
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