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      • 產品名稱：MC 3T3-E1  小鼠成骨細胞 
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      • 產品型號：CRL-2593
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      • 產品廠商：美國標準生物品收藏中心（ATCC）
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      • 產品價格：0
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      • 折扣價格：0
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      • 產品文檔：
                          
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            MC 3T3-E1  小鼠成骨細胞 ，原代細胞|細胞系|細胞株|菌種；細胞庫管理規范，提供的細胞株背景清楚，提供參考文獻和Z優培養條件！
        
      
    
      

    
      
        
      
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            MC 3T3-E1  小鼠成骨細胞 
      

      
	
		
      
			
				ATCC® Number: 
			
			
				CRL-2593?    
			
			
				Price: 
			
			
				$324.00
			
		
      
		
      
			
				
      
					
						
      
							
								Designations: 
							
							
								MC3T3-E1 Subclone 4
							
						
      
						
      
							
								Depositors: 
							
							
								 RT Franceschi 
							
						
      
						
      
							
								Biosafety Level:
							
							
								1
							
						
      
						
      
							
								Shipped: 
							
							
								frozen 
							
						
      
						
      
							
								Medium & Serum: 
							
							
								See Propagation
							
						
      
						
      
							
								Growth Properties:
							
							
								adherent
							
						
      
						
      
							
								Organism:
							
							
								Mus musculus (mouse)
							
						
      
						
      
							
								Morphology:
							
							
								fibroblast
      

      
      
							      		
						
      
						
      
							
								Source:
							
							
								Organ: bone 
      
Strain: C57BL/6 
      
Tissue: calvaria 
      
Cell Type: preosteoblast;      
							      		
						
      
						
      
							
								Cellular Products:
							
							
								collagen [51540]
							
						
      
						
      
							
								Permits/Forms:
							
							
								In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
							
						
      
						
      
							
								 
							
						
      
						
      
							
								Tumorigenic:
							
							
								Yes
							
						
      
						
      
							
								Age: 
							
							
								newborn newborn 
							
						
      
						
      
							
								Comments:
							
							
								A series of subclones were isolated from the cloned but phenotypically heterogeneous MC3T3-E1 cell line. The subclones were selected for high or low osteoblast differentiation and mineralization after growth in medium containing ascorbic acid. The MC3T3-E1 Subclone 4 (ATCC CRL-2593) and the MC3T3 Subclone 14 (ATCC CRL-2594) lines exhibit high levels of osteoblast differentiation after growth in ascorbic acid and 3 to 4 mM inorganic phosphate. They form a well mineralized extracellular matrix (ECM) after 10 days [PubMed ID: 10352097].
      
The MC3T3 Subclone 24 (ATCC CRL-2595) and the MC3T3 Subclone 30 (ATCC CRL-2596) lines exhibit poor osteoblast differentiation after growth in ascorbic acid. They do not form ECM. They can be used as negative controls for Subclones 4 and 14 [PubMed ID: 10352097].
      
Mineralizing subclones selectively express mRNAs for the osteoblast markers, bone sialoprotein (BSP), osteocalcin (OCN), and the parathyroid hormone (PTH)/parathyroid hormone-related protein (PTHrP) receptor.S ubclones with both high and low differentiation potential produce similar amounts of collagen in culture and express comparable basal levels of mRNA encoding Osf2/Cbfa1, an osteoblast-related transcription factor [PubMed ID: 10352097].
      
      
							      		
						
      
					      
				
      
			      		
		
      
	      

      
        
      
    
      

      
 
    
      

      

                            
      
    
      
    
      
        
      

  
      


          
      
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