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      • 產品名稱：MDBK (NBL-1) 牛腎細胞
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      • 產品型號：CCL-22
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      • 產品廠商：美國標準生物品收藏中心（ATCC）
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      • 產品價格：0
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      • 折扣價格：0
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      • 產品文檔：
                          
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            MDBK (NBL-1) 牛腎細胞，原代細胞|細胞系|細胞株|菌種；細胞庫管理規范，提供的細胞株背景清楚，提供參考文獻和培養條件！
        
      
    
      

    
      
        
      
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            MDBK (NBL-1) 牛腎細胞
      

      
	
		
      
			
				ATCC® Number: 
			
			
				CCL-22?    
			
			
				Price: 
			
			
				$289.00
			
		
      
		
      
			
				
      
					
						
      
							
								Designations: 
							
							
								MDBK (NBL-1)
							
						
      
						
      
							
								Depositors: 
							
							
								 S Madin, NB Darby 
							
						
      
						
      
							
								Biosafety Level:
							
							
								1
							
						
      
						
      
							
								Shipped: 
							
							
								frozen 
							
						
      
						
      
							
								Medium & Serum: 
							
							
								See Propagation
							
						
      
						
      
							
								Growth Properties:
							
							
								adherent
							
						
      
						
      
							
								Organism:
							
							
								Bos taurus (cow)
							
						
      
						
      
							
								Morphology:
							
							
								epithelial
      

      
      
							      		
						
      
						
      
							
								Source:
							
							
								Organ: kidney 
      
Disease: normal      
							      		
						
      
						
      
							
								Cellular Products:
							
							
								keratin
							
						
      
						
      
							
								Permits/Forms:
							
							
								In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
							
						
      
						
      
							
								 
							
						
      
						
      
							
								Applications:
							
							
								detection and quantitation of replication competent retroviruses [92434] 
      
transfection host (Nucleofection technology from Lonza)      
							      		
						
      
						
      
							
								Virus Resistance:
							
							
								poliovirus 2
							
						
      
						
      
							
								Cytogenetic Analysis:
							
							
								Chromosome Frequency Distribution 50 Cells: 2n = 60. The stemline chromosome number is hypodiploid with 2S component occurring at 5%., There were a total of 11-14 marker chromosomes (4-5 metacentric, 3-4 submetacentric and 4-5 acro-telocentric) common to most hypodiploid metaphases. The X was monosomic. Neither HSR chromosomes nor DM's were seen.
							
						
      
						
      
							
								Age: 
							
							
								
      
							      		
						
      
						
      
							
								Gender: 
							
							
								male 
							
						
      
						
      
							
								Comments:
							
							
								The MDBK cell line was derived from a kidney of an apparently normal ***** steer, February 18, 1957, by S.H. Madin and N.B. Darby. 
      
It was ascertained in 1973 that the MDBK line was infected with bovine viral diarrhea virus (BVDV). 
      
In 1982 R.A. Van Deusen of the NVSL, USDA, Ames, Iowa informed the ATCC that they had found an MDBK free of BVDV infection. 
      
The cells were originally obtained in July, 1967 at passage 96 from the ATCC as a frozen ampule (F-145). Extensive testing (35 passages) by the NVSL found no evidence of any viral contamination in this particular progeny of MDBK. 
      
The cell line was submitted to the American Type Culture Collection by R.A. Van Deusen in passage 110 in August, 1982. 
      
The cells are positive for keratin by immunofluorescence. 
      
The line has been shown to be free of bovine viral diarrhea virus (BVDV). [22883]      
							      		
						
      
					      
				
      
			      		
		
      
	      

      
        
      
    
      

      
 
    
      

      

                            
      
    
      
    
      
        
      

  
      


          
      
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