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MJ [G11]
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President and Fellows of Harvard College
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Biosafety Level:
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2 [Cells Contain RETROVIRUS ]
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frozen
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See Propagation
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suspension
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Homo sapiens (human)
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lymphoblast
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Organ: peripheral blood
Disease: cutaneous T cell lymphoma; mycosis fungoides
Cell Type: T lymphocyte;
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HTLV-I
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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interleukin 2 (IL-2)
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CD2 +; CD3 +; CD4 +
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Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12,14
D16S539: 11,12
D5S818: 11,13
D7S820: 8,11
THO1: 6,7
TPOX: 8,11
vWA: 15,17
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50 years
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male
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Caucasian
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ATCC complete growth medium: Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
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Protocol: Pellet cells by centrigation, save one third of the spent medium and to two volumes of fresh medium. Resuspend the cell pellet in the mixture and dispense into new flasks. Establish new cultures at about 1 X 10(6) cells/ml.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week
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4002: Essex ME, Lee TH. Method and products for detection of human T cell leukemia virus. US Patent 4,743,678 dated May 10 1988
22945: Popovic M, et al. Isolation and transmission of human retrovirus (human t-cell leukemia virus). Science 219: 856-859, 1983. PubMed: 6600519
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