immunoglobulin; monoclonal antibody; against mouse CD40 ligand (CD154, CD40L, gp39)
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Comments:
Animals were immunized with mouse T cells (Th1).
Spleen cells were fused with NS-1 myeloma cells.
The antibody detects a 39000 dalton protein selectively expressed on activated mouse T cells.
A culture deposited at the ATCC as HB-11048 in May of 1992 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline.
Propagation:
ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10% Temperature: 37.0°C
Subculturing:
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
parental cell line:ATCC HB-11048
References:
23334: Noelle RJ, et al. A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells. Proc. Natl. Acad. Sci. USA 89: 6550-6554, 1992. PubMed: 1378631 23483: Roy M, et al. The regulation of the expression of gp39, the CD40 ligand, on normal and cloned CD4+ T cells. J. Immunol. 151: 2497-2510, 1993. PubMed: 8103067
