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The NG108-15 cell line, originally named 108CC15, was developed in 1971 by Bernd Hamprecht. [112482]
The line was formed by fusing mouse N18TG2 neuroblastoma cells with rat C6-BU-1 glioma cells in the presence of inactivated Sendai virus. [51493]
Propagation:
ATCC complete growth medium: The base medium for this cell line is Dulbecco's Modified Eagle's Medium without sodium pyruvate . To make the complete growth medium, add the following components to the base medium:
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
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