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NTERA-2 cl.D1 [NT2/D1]
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PW Andrews
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Biosafety Level:
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1
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frozen
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See Propagation
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adherent
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Homo sapiens (human)
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epithelial-like,differentiation changes phenotype
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Organ: testis
Disease: malignant pluripotent embryonal carcinoma
Derived from metastatic site: lung
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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transfection host (Roche FuGENE® Transfection Reagents
technology from amaxa)
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RA and HMBA TREATED CELLS: human cytomegalovirus (HCMV); human immunodeficiency virus (HIV-1, HTLV-III)
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UNTREATED CELLS: human cytomegalovirus (HCMV); human immunodeficiency virus (HIV-1, HTLV-III)
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Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 13
D16S539: 11,12,13
D5S818: 9,12
D7S820: 10,12
THO1: 9.3
TPOX: 8
vWA: 18,19
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This is a hypotriploid human cell line with the modal chromosome number of 63 in 48% of cells examined. However, cells with 62 chromosome counts also occurred at a rather high frequency (24%). The rate of polyploidy was 1.6%., About 12 marker chromosomes are constantly found in most cells. They include: der(9)t(1;9)(q25;q34.3); del(1)(q25); der(13)t(11;13)(q13;q34); t(Xq1q); and eight others., At least two markers are found only in some cells. The normal Y chromosome was found in all cells. Only single copies of normal chromosomes 1, 10, 11 and 13 were present. Others were mostly in two or three copies per cell.
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22 years
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male
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Caucasian
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
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Medium Renewal: Every 2 to 3 days
Subcultures are prepared by scraping.
Cells from confluent cultures (approximately 20 million cells per 75 sq. cm.) are dislodged from the flask surface, aspirated and dispensed into new flasks.
Cultures should be maintained at high density.
Seed new flasks at a density of at least 5 X 10 exp6 viable cells per 75 sq. cm. flask.
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culture medium 95%; DMSO, 5%
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Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
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22321: Andrews PW. Human teratocarcinomas. Biochim. Biophys. Acta 948: 17-36, 1988. PubMed: 3293662
22336: Mavilio F, et al. Activation of four homeobox gene clusters in human embryonal carcinoma cells induced to differentiate by retinoic acid. Differentiation 37: 73-79, 1988. PubMed: 2898410
22407: Fenderson BA, et al. Glycolipid core structure switching from globo- to lacto- and ganglio- series during retinoic acid-induced differentiation of TERA-2-derived human embryonal carcinoma cells. Dev. Biol. 122: 21-34, 1987. PubMed: 3297853
22741: Andrews PW, et al. Pluripotent embryonal carcinoma clones derived from the human teratocarcinoma cell line Tera-2. Differentiation in vivo and in vitro. Lab. Invest. 50: 147-162, 1984. PubMed: 6694356
22746: Andrews PW, et al. Different patterns of glycolipid antigens are expressed following differentiation of TERA-2 human embryonal carcinoma cells induced by retinoic acid, hexamethylene bisacetamide (HMBA) or bromodeoxyuridine (BUdR). Differentiation 43: 131-138, 1990. PubMed: 2373286
22947: Gonczol E, et al. Cytomegalovirus replicates in differentiated but not in undifferentiated human embryonal carcinoma cells. Science 224: 159-161, 1984. PubMed: 6322309
22999: Andrews PW. Retinoic acid induces neuronal differentiation of a cloned human embryonal carcinoma cell line in vitro. Dev. Biol. 103: 285-293, 1984. PubMed: 6144603
23235: Hirka G, et al. Differentiation of human embryonal carcinoma cells induces human immunodeficiency virus permissiveness which is stimulated by human cytomegalovirus coinfection. J. Virol. 65: 2732-2735, 1991. PubMed: 1850047
29000: Dewji NN, Singer SJ. Cell surface expression of the Alzheimer disease-related presenilin proteins. Proc. Natl. Acad. Sci. USA 94: 9926-9931, 1997. PubMed: 9275228
49353: Baldassarre G, et al. Transfection with a CRIPTO anti-sense plasmid suppresses endogenous CRIPTO expression and inhibits transformation in a human embryonal carcinoma cell line. Int. J. Cancer 66: 538-543, 1996. PubMed: 8635871
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