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PK136
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GC Koo
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IgG2a
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Biosafety Level:
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1
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frozen
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See Propagation
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suspension
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Mus musculus (B cell); Mus musculus (myeloma) (mouse (B cell); mouse (myeloma))
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lymphoblast
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Cell Type: hybridoma: B lymphocyte;
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immunoglobulin; monoclonal antibody; against mouse natural killer (NK) cells
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
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fetal bovine serum to a final concentration of 10%
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1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
Temperature: 37.0°C
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Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10(5) cells/ml and maintain between 5 X 10(4) and 1 X 10(6) cells/ml. Do not exceed 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
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recommended serum:ATCC 30-2020
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
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1319: Koo GC, et al. The NK-1.1 (-) mouse: a model to study differentiation of murine NK cells. J. Immunol. 137: 3742-3747, 1986. PubMed: 3782794
58145: Koo GC, Peppard JR. Establishment of monoclonal anti-NK-1.1 antibody. Hybridoma 3: 301-303, 1984. PubMed: 6500587
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