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      • 產品名稱：CCL-35 Pt K1 (NBL-3) 袋鼠腎細胞
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      • 產品型號：CCL-35
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      • 產品廠商：美國標準生物品收藏中心（ATCC）
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      • 產品價格：0
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      • 折扣價格：0
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      • 產品文檔：
                          
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            CCL-35 Pt K1 (NBL-3) 袋鼠腎細胞，原代細胞|細胞系|細胞株|菌種；細胞庫管理規范，提供的細胞株背景清楚，提供參考文獻和培養條件！
        
      
    
      

    
      
        
      
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            CCL-35 Pt K1 (NBL-3) 袋鼠腎細胞
      

      
	
		
      
			
				ATCC® Number: 
			
			
				CCL-35?    
			
			
				Price: 
			
			
				$438.00
			
		
      
		
      
			
				
      
					
						
      
							
								Designations: 
							
							
								Pt K1 (NBL-3) [PTK1]
							
						
      
						
      
							
								Depositors: 
							
							
								 KH Walen 
							
						
      
						
      
							
								Biosafety Level:
							
							
								1
							
						
      
						
      
							
								Shipped: 
							
							
								frozen 
							
						
      
						
      
							
								Medium & Serum: 
							
							
								See Propagation
							
						
      
						
      
							
								Growth Properties:
							
							
								adherent
							
						
      
						
      
							
								Organism:
							
							
								Potorous tridactylis (potoroo)
							
						
      
						
      
							
								Morphology:
							
							
								epithelial
      

      
      
							      		
						
      
						
      
							
								Source:
							
							
								Organ: kidney 
      
Disease: normal      
							      		
						
      
						
      
							
								Cellular Products:
							
							
								keratin
							
						
      
						
      
							
								Permits/Forms:
							
							
								In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
							
						
      
						
      
							
								 
							
						
      
						
      
							
								Virus Resistance:
							
							
								poliovirus 2
							
						
      
						
      
							
								Age:
							
							
								
      
							      		
						
      
						
      
							
								Gender: 
							
							
								female 
							
						
      
						
      
							
								Comments:
							
							
								The cells are positive for keratin by immunoperoxidase staining.
							
						
      
						
      
							
								Propagation: 
							
							
								ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids and 1 mM sodium pyruvate, 90%; newborn bovine calf serum, 10%
      
      
							      		
						
      
						
      
							
								Subculturing: 
							
							
								Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended 
      
Medium Renewal: Twice per week 
      
Rinse cell sheet twice with 0.25% trypsin, 0.03% EDTA (or Alsever's Trypsin Versene) solution, remove the trypsin solution, and allow the culture to stand for 5 to 10 minutes at room temperature. Add fresh medium, aspirate and dispense into new flasks.      
							      		
						
      
						
      
							
								References:
							
							
								26188: . . Nature 194: 406, 1962.
      
26189: . . Cytogenetics 3: 19, 1964.      
							      		
						
      
					      
				
      
			      		
		
      
	      

      
        
      
    
      

      
 
    
      

      

                            
      
    
      
    
      
        
      

  
      


          
      
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