|
|
PtK2 (NBL-5)
|
|
|
KH Whalen
|
|
Biosafety Level:
|
1
|
|
|
frozen
|
|
|
See Propagation
|
|
|
adherent
|
|
|
Potorous tridactylis (potoroo)
|
|
|
epithelial
|
|
|
Organ: kidney
Disease: normal
|
|
|
keratin
|
|
|
In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
|
|
|
|
|
transfection host (Roche FuGENE® Transfection Reagents)
|
|
|
adenovirus 5; coxsackievirus B5; poliovirus 2
|
|
|
|
|
|
male
|
|
|
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
|
|
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 daysEvery 2 to 3 days
|
|
|
Freeze medium: growth medium, 95%; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase
|
|
|
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
|
|
|
26208: . . Genetics 51: 915, 1965.
|
