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      • 產(chǎn)品名稱(chēng)：CRL-1581 Sp2/0-Ag14 小鼠骨髓瘤細(xì)胞系
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      • 產(chǎn)品型號(hào)：CRL-1581
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      • 產(chǎn)品廠(chǎng)商：美國(guó)標(biāo)準(zhǔn)生物品收藏中心（ATCC）
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      • 產(chǎn)品價(jià)格：0
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            CRL-1581 Sp2/0-Ag14 小鼠骨髓瘤細(xì)胞系，ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞株|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|；細(xì)胞庫(kù)管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和培養(yǎng)條件
        
      
    
      

    
      
        
      
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            CRL-1581 Sp2/0-Ag14 小鼠骨髓瘤細(xì)胞系
      

      
    
        
      
            ATCC® Number: 
            CRL-1581™    
            Price: 
            $268.00
        
      
        
      
            
            
      
                
                    
      
                        Designations: 
                        Sp2/0-Ag14
                    
      
                    
      
                        Depositors: 
                         G Kohler 
                    
      
                    
      
                        Biosafety Level:
                        1
                    
      
                    
      
                        Shipped: 
                        frozen 
                    
      
                    
      
                        Medium & Serum: 
                        See Propagation
                    
      
                    
      
                        Growth Properties:
                        suspension
                    
      
                    
      
                        Organism:
                        Mus musculus (B cell); Mus musculus (myeloma) (mouse (B cell); mouse (myeloma))
                    
      
                    
      
                        Morphology:
                        lymphoblast
      
                        CRL-1581 Sp2/0-Ag14 小鼠骨髓瘤細(xì)胞系
      
                              		
                    
      
                    
      
                        Source:
                        Organ: spleen 
      
                        Strain: BALB/c 
      
                        Cell Type: hybridoma: B lymphocyte;      		
                    
      
                    
      
                        Permits/Forms:
                        In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
                    
      
                    
      
                         
                    
      
                    
      
                        Applications:
                        fusion partner for production of somatic cell hybrids
                    
      
                    
      
                        Antigen Expression:
                        H-2d
                    
      
                    
      
                        Comments:
                        The line was formed by fusing BALB/c spleen cells (from mouse immunized with sheep RBCs) with the P3X63Ag8 myeloma. [1077] 
      
                        The cells do not secrete immunoglobulin, are resistant to 8-azaguanine at 20 mcg/ml and are HAT sensitive. [1077] 
      
                        Sp2/0-Ag14 cells can be used as fusion partners for B cells in the production of hybridomas. [906] 
      
                        Tested and found negative for ectromelia virus (mousepox).      		
                    
      
                    
      
                        Propagation: 
                        ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
      
                        Temperature: 37.0°C      		
                    
      
                    
      
                        Subculturing: 
                        Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Maintain cell density between 5 X 10 (4) and 5 X 10 (5) viable cells/ml. Some cells can attach and be transferred by shaking them loose. 
      
                        Medium Renewal: Add fresh medium every 2 to 4 days (depending on cell density)      		
                    
      
                    
      
                        Preservation: 
                        Freeze medium: Complete growth medium 95%; DMSO, 5% 
      
                        Storage temperature: liquid nitrogen vapor phase      		
                    
      
                    
      
                        Related Products:
                        Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
      
                        recommended serum:ATCC 30-2020
      
                        derivative:ATCC CRL-1581.1      		
                    
      
                    
      
                        References:
                        906: Ozato K, Sachs DH. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J. Immunol. 126: 317-321, 1981. PubMed: 6935293
      
                        1077: Shulman M, et al. A better cell line for making hybridomas secreting specific antibodies. Nature 276: 269-270, 1978. PubMed: 714156
      
                        32373: Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260
      
                        33167: Chen H, et al. Octamer binding factors and their coactivator can activate the murine PU.1 (spi-1) promoter. J. Biol. Chem. 271: 15743-15752, 1996. PubMed: 8663022      		
                    
      
                      
            
      
                  		
        
      
          

      
        
      
    
      

      
 
    
      

      

                            
      
    
      
    
      
        
      

  
      


          
      
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