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SW 626 [SW-626, SW626]
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A Leibovitz
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Biosafety Level:
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1
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frozen
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See Propagation
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adherent
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Homo sapiens (human)
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epithelial
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Organ: ovary
Tumor Stage: grade III
Disease: adenocarcinoma
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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Isolation date: January, 1974
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Yes
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Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 11
D5S818: 12
D7S820: 10,12
THO1: 9.3
TPOX: 8
vWA: 17,18
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Hypertetraploid; modal number = 104. The rate of higher ploidies was 23%. The markers der(2)t(2;5)(q35;q31); del(8)(q13q22); del(12)(q13); t(q9q13) and two others were common to most cells. Generally there were two copies of der(2) and three copies of del(8) per cell. The t(3;11)(p21;q25) and i(15q) markers were seen in some cells. Many cells had 8 copies of N3, N7, N9, N19 and N20, but only two copies of N2. Normal 8 was absent. There were four copies of X, and Y was not found.
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AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
Me-2, 1
PGM1, 1
PGM3, 1
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46 years
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female
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Caucasian
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air,
Temperature: 37.0°C
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Protocol:
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Remove and discard culture medium.
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Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
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Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
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Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
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Add appropriate aliquots of the cell suspension to new culture vessels.
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Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week
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Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
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Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008
recommended serum:ATCC 30-2020
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22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
32824: Strobel T, et al. BAX enhances paclitaxel-induced apoptosis through a p53-independent pathway. Proc. Natl. Acad. Sci. USA 93: 14094-14099, 1996. PubMed: 8943066
40013: Furlong MT, et al. Evidence for the colonic origin of ovarian cancer cell line SW626. J. Natl. Cancer Inst. 91: 1327-1328, 1999. PubMed: 10433623
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