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      • 產品名稱：CRL-12284 PT67 逆轉錄酶病毒包裝細胞
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      • 產品型號：CRL-12284
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      • 產品廠商：美國標準生物品收藏中心（ATCC）
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      • 產品價格：0
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      • 折扣價格：0
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      • 產品文檔：
                          
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            CRL-12284 PT67 逆轉錄酶病毒包裝細胞，ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞,細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和培養條件
        
      
    
      

    
      
        
      
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            CRL-12284 PT67 逆轉錄酶病毒包裝細胞
      

      
    
        
      
            CRL-12284™    
            Price: 
            $438.00
        
      
        
      
            
            
      
                
                    
      
                        Designations: 
                        PT67 [10A1-based retrovirus packaging cell line]
                    
      
                    
      
                        Depositors: 
                         Fred Hutchinson Cancer Res. Cntr. 
                    
      
                    
      
                        Biosafety Level:
                        2 [cells contain SV-40 viral DNA sequences ]
                    
      
                    
      
                        Shipped: 
                        frozen 
                    
      
                    
      
                        Medium & Serum: 
                        See Propagation
                    
      
                    
      
                        Growth Properties:
                        adherent
                    
      
                    
      
                        Organism:
                        Mus musculus (mouse)
                    
      
                    
      
                        Morphology:
                        
      
                              		
                    
      
                    
      
                        Permits/Forms:
                        In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
                    
      
                    
      
                         
                    
      
                    
      
                        Applications:
                        transfection host (Roche FuGENE® Transfection Reagents)
                    
      
                    
      
                        Comments:
                        PT67 is a retrovirus packaging cell line derived from TK- NIH/3T3 cells. 
      
                        The cells produce replication-defective retroviral vector particles capable of binding to the gibbon ape leukemia virus receptor Glvr-1 (Pit-1) or the amphotropic retrovirus receptor Ram-1 (Pit-2) on target cells. [32364]      		
                    
      
                    
      
                        Propagation: 
                        ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
      
                        Temperature: 37.0°C 
      
                        Atmosphere: air, 95%; carbon dioxide (CO2), 5%      		
                    
      
                    
      
                        Subculturing: 
                        Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:12 is recommended 
      
                        Medium Renewal: Every 2 to 3 days 
      
                        Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. 
      
                        Add fresh culture medium, aspirate and dispense into new culture flasks.      		
                    
      
                    
      
                        Preservation: 
                        culture medium 95%; DMSO, 5%
                    
      
                    
      
                        Related Products:
                        Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
      
                        recommended serum:ATCC 30-2020      		
                    
      
                    
      
                        References:
                        32364: Miller AD, Chen F. Retrovirus packaging cells based on 10AI murine leukemia virus for production of vectors that use multiple receptors for cell entry. J. Virol. 70: 5564-5571, 1996. PubMed: 8764070
      
                        46921: Miller AD. 10A1 Retroviral packaging cells and uses thereof. US Patent 5,766,945 dated Jun 16 1998      		
                    
      
                      
            
      
                  		
        
      
          

      
        
      
    
      

      
 
    
      

      

                            
      
    
      
    
      
        
      

  
      


          
      
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