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      產(chǎn)品詳情
      • 產(chǎn)品名稱:人**內(nèi)膜腺癌細(xì)胞

      • 產(chǎn)品型號:HTB-111
      • 產(chǎn)品廠商:美國標(biāo)準(zhǔn)生物品收藏中心(ATCC)
      • 產(chǎn)品價(jià)格:0
      • 折扣價(jià)格:0
      • 產(chǎn)品文檔:
      你添加了1件商品 查看購物車
      簡單介紹:
      HTB-111 AN3 CA 人**內(nèi)膜腺癌細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!
      詳情介紹:
      HTB-111 AN3 CA 人**內(nèi)膜腺癌細(xì)胞
      ATCC® Number: HTB-111?    Price: $399.00
      Designations: AN3 CA
      Depositors:  CJ Dawe
      Biosafety Level: 1
      Shipped: frozen
      Medium & Serum: See Propagation
      Growth Properties: adherent
      Organism: Homo sapiens (human)
      Morphology: epithelial

      Source: Organ: uterus
      Tissue: endometrium
      Disease: adenocarcinoma
      Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
       
      Tumorigenic: Yes
      DNA Profile (STR): Amelogenin: X
      CSF1PO: 12,14,15
      D13S317: 12,14
      D16S539: 10,14
      D5S818: 11,14
      D7S820: 7,10,7.1
      THO1: 10,9.3
      TPOX: 8,10
      vWA: 14,20
      Isoenzymes: AK-1, 1-2
      ES-D, 1
      G6PD, B
      GLO-I, 2
      PGM1, 1
      PGM3, 1-2
      Age: 55 years
      Gender: female
      Ethnicity: Caucasian
      Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
      Atmosphere: air, 95%; carbon dioxide (CO2), 5%
      Temperature: 37.0°C
      Subculturing: Protocol: Volumes used in this protocol are for 75 sq. cm flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
      1. Remove and discard culture medium.
      2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
      3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
        Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
      4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
      5. Add appropriate aliquots of the cell suspension to new culture vessels.
      6. Incubate cultures at 37C.

      Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
      Medium Renewal: 2 to 3 times per week
      Preservation: Freeze medium: Culture medium, 95%; DMSO, 5%
      Storage temperature: liquid nitrogen vapor phase
      Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
      recommended serum:ATCC 30-2020
      References: 22517: Dawe CJ, et al. Growth in continuous culture, and in hamsters, of cells from a neoplasma assoicated with Acanthosis nigricans. J. Natl. Cancer Inst. 33: 441-456, 1964. PubMed: 14207855
      22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
      29988: Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105
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