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Aspergillus niger van Tieghem, anamorph
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NRRL 330 [4050, CBS 122.49, CECT 2775, IFO 6661, IMI 60286, MUCL 28817, WB 330]
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Chinese galls
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NRRL
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ATCC <<--NRRL<<--C. Thom 4050 <<--- Eastman Kodak Co.
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Biosafety Level:
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1
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freeze-dried
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ATCC medium 325: Malt extract agar (Blakeslee's formula)
Temperature: 24.0°C
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degrades unhydrolyzed potato starch [860]
produces acetyl-xylan esterase [58340]
produces acetylesterase acetyl esterase [18117]
produces alpha-glucosidase maltase
produces amylase, alpha 1A; salivary Amylase, salivary, alpha-1A
produces ethyl alcohol ethanol [860]
produces glucoamylase [46568]
produces monoamine oxidase [17694]
produces saccharifying enzymes [52819]
transformation host [13801] [15113] [16756]
produces large amounts of maltase and smaller amounts of alpha-amylase [49287]
produces ethanol from potato starch when cocultured with Saccharomyces cerevisiae ATCC 26603 [860]
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Fungi
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860: Abouzied MM, Reddy CA. Direct fermentation of potato starch to ethanol by cocultures of Aspergillus niger and Saccharomyces cerevisiae. Appl. Environ. Microbiol. 52: 1055-1059, 1986. PubMed: 3539016
13801: Mohr G, Esser K. Improved transformation frequency and heterologous promoter recognition in Aspergillus niger. Appl. Microbiol. Biotechnol. 34: 63-70, 1990. PubMed: 1366974
15113: Brandhorst T, Kenealy WR. Effects of leader sequences upon the heterologous expression of restrictocin in Aspergillus nidulans and Aspergillus niger. Can. J. Microbiol. 41: 601-611, 1995. PubMed: 7641142
16756: . . DECHEMA Biotechnol. Conf. 3A: 319-323, 1989.
17694: Hoover LK, et al. Biotransformation of dopamine to norlaudanosoline by Aspergillus niger. Biotechnol. Bioeng. 38: 1029-1033, 1991.
18117: Linden J, et al. Purification and characterization of an acetyl esterase from Aspergillus niger. Appl. Biochem. Biotechnol. 45-46: 383-393, 1994. PubMed: 8010767
46568: Pazur JH, et al. Glycoenzymes: Structure and properties of the two forms of glucoamylase from Aspergillus niger. Carbohydr. Res. 20: 83-96, 1971. PubMed: 5151200
49287: Dingle J, Solomons GL. Enzymes from micro-fungi. J. Appl. Chem. 2: 395-399, 1952.
52819: Corman J, Langlykke AF. Action of mold enzymes in starch saccharification. Cereal Chem. 25: 190-201, 1948.
58340: Khan AW, et al. Comparison of natural hemicellulose and chemically acetylated xylan as substrates for the determination of acetyl-xylan esterase activity in Aspergilli. Enzyme Microb. Technol. 12: 127-131, 1990.
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