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      產品詳情
      • 產品名稱: MC/CAR

      • 產品型號:CRL-8083
      • 產品廠商:美國標準生物品收藏中心(ATCC)
      • 產品價格:0
      • 折扣價格:0
      • 產品文檔:
      你添加了1件商品 查看購物車
      簡單介紹:
      CRL-8083 MC/CAR 人外周血LIN巴細胞,ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|;細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和培養條件!
      詳情介紹:
      CRL-8083 MC/CAR 人外周血**細胞
      ATCC® Number: CRL-8083?    Price: $417.00
      Designations: MC/CAR
      Depositors:  Research Corporation
      Isotype: IgG1 (cytoplasmic); kappa light chain
      Biosafety Level: 2
      Shipped: frozen
      Medium & Serum: See Propagation
      Growth Properties: suspension
      Organism: Homo sapiens (human)
      Morphology: lymphoblast

      Source: Organ: peripheral blood
      Disease: plasmacytoma; myeloma
      Cell Type: B lymphocyte;
      Cellular Products: immunoglobulin
      Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
       
      Receptors: complement (C3b)
      Tumorigenic: Yes
      Antigen Expression: HLA A1, A33/31, B7, B18, DRw1, DRw2; CD11a +; CD19 +; CD20 + CD28 -; CD38 +
      DNA Profile (STR): Amelogenin: X,Y
      CSF1PO: 11,12
      D13S317: 11,13
      D16S539: 11,12
      D5S818: 11,14
      D7S820: 9,13
      THO1: 6,9
      TPOX: 8
      vWA: 17,18
      Cytogenetic Analysis: normal male diploid
      Isoenzymes: ADA, 1
      AK-1, 1
      ES-D, 1
      G6PD, B
      GLO-I, 1
      PGM1, 1
      PGM3, 1
      Age: 81 years
      Gender: male
      Ethnicity: Caucasian
      Comments: Although derived from cells taken from a patient with a plasmacytoma, this line has been shown to be an EBV-transformed B lymphoblastoid cell line. [43270]
      The cells produce, but do not secrete, immunoglobulin.
      No surface immunoglobulin.
      8-azaguanine mutants may be cloned out or mutagenized for HAT sensitive cultures.
      Can be used as a human fusion line.
      Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20%
      Temperature: 37.0°C
      Subculturing: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp5 cells/ml and maintain between 5 X 10 exp5 and 1 X 10 exp6 cells/ml.
      References: 3535: Ritts RE Jr.. Human nonsecretory plasmacytoid cell line. US Patent 4,434,230 dated Feb 28 1984
      43268: Pellat-Deceunynk C, et al. Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after. Blood 86: 4001-4002, 1995. PubMed: 7579375
      43270: Drexler H, et al. False human hematopoietic cell lines: cross-contaminations and misinterpretations. Leukemia 13: 1601-1607, 1999. PubMed: 10516762
      58062: Ritts RE J, et al. Establishment and characterization of a human non-secretory plasmacytoid cell line and its hybridization with human B cells. Int. J. Cancer 31: 133-141, 1983. PubMed: 6600719
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