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Karyotype unstable within stemline range (78-81). Approximately 21% of the cells contained an extra large chromosome with a terminal centromere and 21% contained minute chromosomes.
Age:
embryo
Comments:
The 3T6 cell line is a collagen and hyaluronic acid secreting line established by G. Todaro and H. Green in 1963 from disaggregated Swiss mouse embryos. [5732]
Tested and found negative for ectromelia virus (mousepox).
Propagation:
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C
Subculturing:
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation:
culture medium 95%; DMSO, 5%
Related Products:
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References:
5732: Todaro GJ, Green H. Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J. Cell Biol. 17: 299-313, 1963. PubMed: 13985244 26261: Vogt M, Dulbecco R. Studies on cells rendered neoplastic by polyoma virus: the problem of the presence of virus-related materials. Virology 16: 41-51, 1962. PubMed: 13926482 26270: Green H, et al. Differentiated cell types and the regulation of collagen synthesis. Nature 212: 631-633, 1966. PubMed: 5971697
