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Designations:
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SW 982 [SW-982, SW982]
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Depositors:
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A Leibovitz
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Biosafety Level:
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1
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Shipped:
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frozen
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Medium & Serum:
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See Propagation
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Growth Properties:
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adherent
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Organism:
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Homo sapiens
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Morphology:
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mixed
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Source:
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Tissue: synovium
Disease: synovial sarcoma
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Permits/Forms:
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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DNA Profile (STR):
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Amelogenin: X
CSF1PO: 11,12
D13S317: 12,13
D16S539: 11,12
D5S818: 11,13
D7S820: 9,11
THO1: 9.3
TPOX: 9,11
vWA: 19,20
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Cytogenetic Analysis:
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Hyperdiploid; modal number = 48; range = 42 to 58. The rate of higher ploidies was 1.6%. Nine markers were common to all cells. These were: t(1q4p), del(5) (q31;q33), der(9) t(4;9) (q11;p24), t(8q12p), t(9q13q) and four others.Double minutes (DM) were seen in some cells (usually only one copy). Normal N9 was absent, N4, N8, and N13 were consistently single-copied and the X was double-copied.
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Isoenzymes:
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AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
PGM1, 1-2
PGM3, 1-2
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Age:
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25 years
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Gender:
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female
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Ethnicity:
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Caucasian
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Propagation:
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 100%
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Subculturing:
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Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
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Preservation:
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culture medium 95%; DMSO, 5%
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Related Products:
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Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008
recommended serum:ATCC 30-2020
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References:
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22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
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