2 [Cells contain HPV-16 and c-Ha-ras viral DNA sequences ]
Shipped:
frozen
Medium & Serum:
See Propagation
Growth Properties:
adherent
Organism:
Mus musculus
Morphology:
epithelial
CRL-2785 TC-1 小鼠肺上皮細胞
Source:
Organ: lung Strain: C57BL/6 Disease: tumor Cell Type: human papillomavirus 16 (HPV-16) E6/E7 and c-Ha-Ras cotransformed
Permits/Forms:
In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:
Part of the Johns Hopkins Special Collection
Applications:
The TC-1 lung metastasis model can be used to test the efficacy of various E6/E7-specific vaccines and immunotherapeutic strategies [PubMed: 9724092].
The tumor cell line, TC-1 [tissue culture number one], was derived from primary lung epithelial cells of C57BL/6 mice.
The cells are positive for the expression of HPV-16 E7 [PubMed: 8548765].
Tumorigenic:
Yes CRL-2785 TC-1 小鼠肺上皮細胞
Comments:
The tumor cell line, TC-1 [tissue culture number one], was derived from primary lung epithelial cells of C57BL/6 mice. The cells were immortalized with the amphotropic retrovirus vector LXSN16E6E7 and subsequently transformed with the pVEJB plasmid expressing the activated human c-Ha-ras oncogene [PubMed: 8548765]. The transformed cells were selected with G418 and Hygromycin B [PubMed: 8548765]. The cells are positive for the expression of HPV-16 E7 [PubMed: 8548765].The TC-1 lung metastasis model can be used to test the efficacy of various E6/E7-specific vaccines and immunotherapeutic strategies [PubMed: 9724092].
Propagation:
ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate supplemented with 0.1 mM nonessential amino acids, 90%; fetal bovine serum, 10% Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
Subculturing:
Protocol: Subculture before confluence.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Cultures can be established between 1 X 10(5) and 2 X 10(5) viable cells/cm2.Maintain cultures at a cell concentration between 2 X 10(5) and 4 X 10(5) viable cells/cm2. Do not exceed 6 X 10(5) cells/cm2..
Incubate cultures at 37°C.
Interval: A subcultivation ratio of 1:3 to 1:10 is recommended Medium Renewal: Every 2 to 3 days
Preservation:
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Doubling Time:
24 hours
Related Products:
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
source culture:ATCC JHU-1
References:
57430: Lin KY, et al. Treatment of established tumors with a novel vaccine that enhances major histocompatibility class II presentation of tumor antigen. Cancer Res. 56: 21-26, 1996. PubMed: 8548765 88870: Chen CH, et al. Antigen-specific immunotherapy for human papillomavirus 16 E7-expressing tumors grown in the liver. J. Hepatol. 33: 91-98, 2000. PubMed: 10905591 88871: Ji H, et al. Antigen-specific immunotherapy for murine lung metastatic tumors expressing human papillomavirus type 16 E7 oncoprotein. Int. J. Cancer 78: 41-45, 1998. PubMed: 9724092
